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KMID : 0375319920140020211
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Journal of Clinical Pathology and Quality Control
1992 Volume.14 No. 2 p.211 ~ p.214
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Effect of Time Elapsed after Collection on Blood Ammonia Measurement
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Choi Jong-Rak
Kim-Jeong-Ho
Song Kyoung-Soon
Kwon Oh-Hyun
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Abstract
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We evaluated new Ammonia Checker ~´ (Kyoto Daichii, Japan), comparing with the Ammonia Checker I® previously used. Both instruments are same in principle which apply the microdiffusion of reagent strip and reflectance photometer using 20,u L of whole blood. The only difference between these checkers is the analysis time intervals, shortening to 3 mina by checker II from 15 by Checker I . The regression analysis on the results from these two instruments showed rather poor correlation with narrow assay range, but which seems to be clinically acceptable (y = 0.79x + 22.4, r = 0.722, p = 0.003, n=20).
We observed the changes of reference range of blood ammonia level by the new instrument, according to the time intervals from the sample collection to the measurement. The average increments of blood ammonia concentration were 8.5%, 23.2%, 58.6% at 30 minutes, at 1 hour, and at 2 hours respectively comparing with the results determined within 10 minutes after blood sampling. All these increments were statistically significant (p<0.01). So we regarded the blood ammonia reference range as the results (19.7-72.7 ,ag/dL) determined 1 hour after blood sampling, considering the usual average time passed after blood drawing until the measurement.
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KEYWORD
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Blood ammonia, Microdiffusion method, Reflectance photometer
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